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4bc16de163 QUIZ. The latter stands for the appearance of the product P in solution (+ d[P]/dt) whose phenomenological rate equation (first-order) is given by . Before proceeding, one should state (and remember) some implicite assumptions: As long as initial velocity is considered, the concentration of product can be neglected (compared to that of the substrate, thus [P] (11) . Last update: Dec 1999- Claude Aflalo You are visitor No. Note that [S] here represents the free substrate concentration, but typically is assumed to be close to the total substrate concentration present in the system.
Van Slyke, D. We know that the free enzyme concentration [E] is equal to the total enzyme concentration [ET] minus [ES]. Note that kon has units of concentration-1time-1, and koff and kcat have units of time-1. D., and Cullen, G. For other suggestions on how to make your study of biochemistry easier, see Learning Strategies. A simple model of enzyme action:. This is also the reason that well-designed experiments measure reaction velocity only in regimes where product formation is linear with time.